Under the tutelage of his father, Masahiro was first an actor, appearing in his first film at the tender age of 3, and starred with eventual director Tomu Uchida in Kusu Kichiko, Sakurai no Ketsubetsu (1921). After graduating from school in 1925, he officially joined Makino Film Productions as an actor/assistant director. His debut as a director was Aoi Me no Ningyo (1926). He then teamed up with screenplay writer Itaro Sanjo and cinematographer Minoru Niki to create Roninkai - Daiichiwa Utkushiki Emono (1928) and Kubi no Za (1929), which won consecutive #1 rankings in the movie Top Ten. Each of these period films produced by the "Young Makino Trio" while Makino was still in his early twenties was revolutionary and caused a sensation. After father Shozo's death, Makino Film Productions went bankrupt and Makino joined Nikkatsu, where he worked on period films starring Tsumasaburo Bando and Chiezo Kataoka. After World War II, he made many movies with chivalrous themes at Toho and Toei studios. In 1993, he died from chronic breathing difficulties.